Background: Currently,the blood glucose control in women with gestational diabetes (GDM) in Vietnam has been still based on the criteria applied for non-pregnant diabetic patients, while these GDM patients require more specific criteria in order to get a better care of the women and the good delivery outcome. Objective: To determine serum fructosamine target as a supporting test for the treatment and follow-up of GDM. Methodology: A prospective study was done in 451women with GDM at Cho Ray Hospital from August 2016 to March 2019.At least 2 of 3 tests (Go, G2 and HbA1c) and serum fructosamine were determined every 2-3 week-up until delivery. Result: The target fructosamine reference value at Cho Ray Hospital was 188.5±42.3µmol/L. Conclusion: The specific target of serum fructosamine in GDM treatment at Cho Ray hospital should be £188.5 ± 42.3mmol/L.